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Bracken

Bracken (Bayesian Reestimation of Abundance with KrakEN) is a highly accurate statistical method that computes the abundance of species in DNA sequences from a metagenomics sample. Bracken uses the taxonomy labels assigned by Kraken, a highly accurate metagenomics classification algorithm, to estimate the number of reads originating from each species present in a sample.

Profile Format

The following input format is accepted by taxpasta. This file is generated with the Bracken parameter -o. A tab-separated file with the following column headers:

Column Header Description
name
taxonomy_id
taxonomy_lvl
kraken_assigned_reads
added_reads
new_est_reads
fraction_total_reads

Example

name    taxonomy_id taxonomy_lvl    kraken_assigned_reads   added_reads new_est_reads   fraction_total_reads
Homo sapiens    9606    S   156 0   156 0.90173
Saccharomyces cerevisiae    4932    S   17  0   17  0.09827